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Parallel CRISPR-Cas9 screens clarify impacts of p53 on screen performance

CRISPR-Cas9 genome engineering has revolutionised high-throughput functional genomic screens. However, recent work has raised concerns regarding the performance of CRISPR-Cas9 screens using TP53 wild-type human cells due to a p53-mediated DNA damage response (DDR) limiting the efficiency of generati...

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Publicat a:eLife
Autors principals: Bowden, Anne Ramsay, Morales-Juarez, David A, Sczaniecka-Clift, Matylda, Agudo, Maria Martin, Lukashchuk, Natalia, Thomas, John Christopher, Jackson, Stephen P
Format: Artigo
Idioma:Inglês
Publicat: eLife Sciences Publications, Ltd 2020
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7244323/
https://ncbi.nlm.nih.gov/pubmed/32441252
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.55325
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