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Characterizing Single-Molecule Conformational Changes Under Shear Flow with Fluorescence Microscopy
Single-molecule behavior under mechanical perturbation has been characterized widely to understand many biological processes. However, methods such as atomic force microscopy have limited temporal resolution, while Förster resonance energy transfer (FRET) only allow conformations to be inferred. Flu...
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| Publicado no: | J Vis Exp |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7205595/ https://ncbi.nlm.nih.gov/pubmed/32065139 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/60784 |
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