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In Vivo Expression of Mutant Calpains in the Eye Using Lentivirus
Exome sequencing has identified many candidate genes and mutations for human diseases, but the functional validation of these candidates is a time-consuming and costly process. Here, we describe a method which uses lentiviruses to overexpress calpain mutations that may play a role in dominant diseas...
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| Publicado no: | Methods Mol Biol |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7173352/ https://ncbi.nlm.nih.gov/pubmed/30617808 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-8988-1_18 |
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