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In Vivo Expression of Mutant Calpains in the Eye Using Lentivirus

Exome sequencing has identified many candidate genes and mutations for human diseases, but the functional validation of these candidates is a time-consuming and costly process. Here, we describe a method which uses lentiviruses to overexpress calpain mutations that may play a role in dominant diseas...

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Bibliografiske detaljer
Udgivet i:Methods Mol Biol
Main Authors: Wert, Katherine J., Mahajan, Vinit B.
Format: Artigo
Sprog:Inglês
Udgivet: 2019
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7173352/
https://ncbi.nlm.nih.gov/pubmed/30617808
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-8988-1_18
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