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Expression in Escherichia coli and purification of biologically active L proteinase of foot-and-mouth disease virus
The foot-and-mouth disease virus (FMDV) Lb gene was cloned into bacterial expression vectors under the control of a T7 RNA polymerase promoter. The Lb protein was expressed in both an in vitro transcription-translation system and in Escherichia coli. In vitro expression of a construct containing the...
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| Veröffentlicht in: | Virus Res |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Published by Elsevier B.V.
1995
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7172946/ https://ncbi.nlm.nih.gov/pubmed/7785315 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/0168-1702(94)00084-P |
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