Improved production of recombinant Rhizomucor miehei lipase by coexpressing protein folding chaperones in Pichia pastoris, which triggered ER stress

Rhizomucor miehei lipase (RML) is a biocatalyst that widely used in laboratory and industrial. Previously, RML with a 70-amino acid propeptide (pRML) was cloned and expressed in P. pastoris. Recombinant strains with (strain containing 4-copy prml) and without ER stress (strain containing 2-copy prml...

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Detalhes bibliográficos
Publicado no:Bioengineered
Main Authors: Huang, Jinjin, Zhao, Qingyi, Chen, Lingxiao, Zhang, Chunmei, Bu, Wei, Zhang, Xin, Zhang, Kaini, Yang, Zhen
Formato: Artigo
Idioma:Inglês
Publicado em: Taylor & Francis 2020
Assuntos:
Research Paper
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7161542/
https://ncbi.nlm.nih.gov/pubmed/32175802
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/21655979.2020.1738127
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