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Structure-Guided Modification of Rhizomucor miehei Lipase for Production of Structured Lipids

To improve the performance of yeast surface-displayed Rhizomucor miehei lipase (RML) in the production of human milk fat substitute (HMFS), we mutated amino acids in the lipase substrate-binding pocket based on protein hydrophobicity, to improve esterification activity. Five mutants: Asn87Ile, Asn87...

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Detalhes bibliográficos
Main Authors: Zhang, Jun-Hui, Jiang, Yu-Yan, Lin, Ying, Sun, Yu-Fei, Zheng, Sui-Ping, Han, Shuang-Yan
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3700896/
https://ncbi.nlm.nih.gov/pubmed/23844120
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0067892
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