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Structure-Guided Modification of Rhizomucor miehei Lipase for Production of Structured Lipids
To improve the performance of yeast surface-displayed Rhizomucor miehei lipase (RML) in the production of human milk fat substitute (HMFS), we mutated amino acids in the lipase substrate-binding pocket based on protein hydrophobicity, to improve esterification activity. Five mutants: Asn87Ile, Asn87...
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| Main Authors: | , , , , , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Public Library of Science
2013
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3700896/ https://ncbi.nlm.nih.gov/pubmed/23844120 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0067892 |
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