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Structure-Guided Modification of Rhizomucor miehei Lipase for Production of Structured Lipids
To improve the performance of yeast surface-displayed Rhizomucor miehei lipase (RML) in the production of human milk fat substitute (HMFS), we mutated amino acids in the lipase substrate-binding pocket based on protein hydrophobicity, to improve esterification activity. Five mutants: Asn87Ile, Asn87...
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| Hauptverfasser: | , , , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Public Library of Science
2013
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3700896/ https://ncbi.nlm.nih.gov/pubmed/23844120 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0067892 |
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