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Improved production of a recombinant Rhizomucor miehei lipase expressed in Pichia pastoris and its application for conversion of microalgae oil to biodiesel

BACKGROUND: We previously cloned a 1,3-specific lipase gene from the fungus Rhizomucor miehei and expressed it in methylotrophic yeast Pichia pastoris strain GS115. The enzyme produced (termed RML) was able to catalyze methanolysis of soybean oil and showed strong position specificity. However, the...

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書誌詳細
出版年:Biotechnol Biofuels
主要な著者: Huang, Jinjin, Xia, Ji, Yang, Zhen, Guan, Feifei, Cui, Di, Guan, Guohua, Jiang, Wei, Li, Ying
フォーマット: Artigo
言語:Inglês
出版事項: BioMed Central 2014
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC4364654/
https://ncbi.nlm.nih.gov/pubmed/25788976
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1754-6834-7-111
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