Linker and N-Terminal Domain Engineering of Pyrrolysyl-tRNA Synthetase for Substrate Range Shifting and Activity Enhancement

The Methanosarcina mazei pyrrolysyl-tRNA synthetase (PylRS)⋅tRNA(Pyl) pair can be used to incorporate non-canonical amino acids (ncAAs) into proteins at installed amber stop codons. Although engineering of the PylRS active site generates diverse binding pockets, the substrate ranges are found simila...

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Detalles Bibliográficos
Publicado en:Front Bioeng Biotechnol
Autores principales: Jiang, Han-Kai, Lee, Man-Nee, Tsou, Jo-Chu, Chang, Kuan-Wen, Tseng, Hsueh-Wei, Chen, Kuang-Po, Li, Yaw-Kuen, Wang, Yane-Shih
Formato: Artigo
Lenguaje:Inglês
Publicado: Frontiers Media S.A. 2020
Materias:
Bioengineering and Biotechnology
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC7156790/
https://ncbi.nlm.nih.gov/pubmed/32322577
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fbioe.2020.00235
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