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Enzymatic Assembly of Overlapping DNA Fragments
Three methods for assembling multiple, overlapping DNA molecules are described. Each method shares the same basic approach: (i) an exonuclease removes nucleotides from the ends of double-stranded (ds) DNA molecules, exposing complementary single-stranded (ss) DNA overhangs that are specifically anne...
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| Publicado no: | Methods Enzymol |
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| Autor principal: | |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Elsevier Inc.
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7149801/ https://ncbi.nlm.nih.gov/pubmed/21601685 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/B978-0-12-385120-8.00015-2 |
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