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Enzymatic Assembly of Overlapping DNA Fragments

Three methods for assembling multiple, overlapping DNA molecules are described. Each method shares the same basic approach: (i) an exonuclease removes nucleotides from the ends of double-stranded (ds) DNA molecules, exposing complementary single-stranded (ss) DNA overhangs that are specifically anne...

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Dettagli Bibliografici
Pubblicato in:Methods Enzymol
Autore principale: Gibson, Daniel G.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Elsevier Inc. 2011
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7149801/
https://ncbi.nlm.nih.gov/pubmed/21601685
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/B978-0-12-385120-8.00015-2
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