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Reversible biotinylation of purified proteins for measuring protein–protein interactions
Measuring protein–protein interactions using purified proteins in vitro is one of the most frequently used approach to understand the biochemical and mechanistic details of cellular signaling pathways. Typically, affinity tags are genetically fused to proteins of interest, and they are used to captu...
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| Publicado en: | Methods Enzymol |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2020
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7115918/ https://ncbi.nlm.nih.gov/pubmed/32046851 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2019.11.008 |
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