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Effective CRISPRa-mediated control of gene expression in bacteria must overcome strict target site requirements

In bacterial systems, CRISPR-Cas transcriptional activation (CRISPRa) has the potential to dramatically expand our ability to regulate gene expression, but we lack predictive rules for designing effective gRNA target sites. Here, we identify multiple features of bacterial promoters that impose strin...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Nat Commun
Hauptverfasser: Fontana, Jason, Dong, Chen, Kiattisewee, Cholpisit, Chavali, Venkata P., Tickman, Benjamin I., Carothers, James M., Zalatan, Jesse G.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Publishing Group UK 2020
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7113249/
https://ncbi.nlm.nih.gov/pubmed/32238808
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-020-15454-y
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