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Effective CRISPRa-mediated control of gene expression in bacteria must overcome strict target site requirements
In bacterial systems, CRISPR-Cas transcriptional activation (CRISPRa) has the potential to dramatically expand our ability to regulate gene expression, but we lack predictive rules for designing effective gRNA target sites. Here, we identify multiple features of bacterial promoters that impose strin...
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| Publicado no: | Nat Commun |
|---|---|
| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group UK
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7113249/ https://ncbi.nlm.nih.gov/pubmed/32238808 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-020-15454-y |
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