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Assessing the efficacy of CRISPR/Cas9 genome editing in the wheat pathogen Parastagonspora nodorum

BACKGROUND: The genome-editing tool CRISPR/Cas9 has revolutionized gene manipulation by providing an efficient method to generate targeted mutations. This technique deploys the Cas9 endonuclease and a guide RNA (sgRNA) which interact to form a Cas9-sgRNA complex that initiates gene editing through t...

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Detalhes bibliográficos
Publicado no:Fungal Biol Biotechnol
Main Authors: Khan, Haseena, McDonald, Megan C., Williams, Simon J., Solomon, Peter S.
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7110818/
https://ncbi.nlm.nih.gov/pubmed/32257291
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s40694-020-00094-0
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