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Handling and Assessment of Human Primary Prostate Organoid Culture

This paper describes a detailed protocol for three-dimensional (3D) culturing, handling, and evaluation of human primary prostate organoids. The process involves seeding of epithelial cells sparsely in a 3D matrix gel on a 96-well microplate with media changes to cultivate expansion into organoids....

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Bibliografiske detaljer
Udgivet i:J Vis Exp
Main Authors: McCray, Tara, Richards, Zachary, Marsili, Joseph, Prins, Gail S., Nonn, Larisa
Format: Artigo
Sprog:Inglês
Udgivet: 2019
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7103137/
https://ncbi.nlm.nih.gov/pubmed/30735176
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/59051
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