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Handling and Assessment of Human Primary Prostate Organoid Culture
This paper describes a detailed protocol for three-dimensional (3D) culturing, handling, and evaluation of human primary prostate organoids. The process involves seeding of epithelial cells sparsely in a 3D matrix gel on a 96-well microplate with media changes to cultivate expansion into organoids....
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| Publicado en: | J Vis Exp |
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| Autores principales: | , , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
2019
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7103137/ https://ncbi.nlm.nih.gov/pubmed/30735176 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/59051 |
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