A python script to design site‐directed mutagenesis primers

Primer design is essential to conduct whole plasmid site‐directed mutagenesis for protein study. Traditionally, primers of mutagenesis are designed manually that is time‐consuming and fallible. Here, we present a Python script for searching primers by presetting parameters of nucleotide composition...

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Detalhes bibliográficos
Publicado no:Protein Sci
Main Authors: Bi, Chuyun, Huang, Xiaofang, Tang, Dan, Shi, Yuanyuan, Zhou, Lixiang, Hu, Yunzhuo, Chen, Xuanyang, Qi, Shiqian, Lin, Shiqiang
Formato: Artigo
Idioma:Inglês
Publicado em: John Wiley & Sons, Inc. 2019
Assuntos:
Tools for Protein Science
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7096704/
https://ncbi.nlm.nih.gov/pubmed/31855299
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.3806
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