A python script to design site‐directed mutagenesis primers

Primer design is essential to conduct whole plasmid site‐directed mutagenesis for protein study. Traditionally, primers of mutagenesis are designed manually that is time‐consuming and fallible. Here, we present a Python script for searching primers by presetting parameters of nucleotide composition...

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Bibliografski detalji
Izdano u:Protein Sci
Glavni autori: Bi, Chuyun, Huang, Xiaofang, Tang, Dan, Shi, Yuanyuan, Zhou, Lixiang, Hu, Yunzhuo, Chen, Xuanyang, Qi, Shiqian, Lin, Shiqiang
Format: Artigo
Jezik:Inglês
Izdano: John Wiley & Sons, Inc. 2019
Teme:
Tools for Protein Science
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7096704/
https://ncbi.nlm.nih.gov/pubmed/31855299
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.3806
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