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A python script to design site‐directed mutagenesis primers

Primer design is essential to conduct whole plasmid site‐directed mutagenesis for protein study. Traditionally, primers of mutagenesis are designed manually that is time‐consuming and fallible. Here, we present a Python script for searching primers by presetting parameters of nucleotide composition...

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書目詳細資料
發表在:Protein Sci
Main Authors: Bi, Chuyun, Huang, Xiaofang, Tang, Dan, Shi, Yuanyuan, Zhou, Lixiang, Hu, Yunzhuo, Chen, Xuanyang, Qi, Shiqian, Lin, Shiqiang
格式: Artigo
語言:Inglês
出版: John Wiley & Sons, Inc. 2019
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC7096704/
https://ncbi.nlm.nih.gov/pubmed/31855299
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.3806
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