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Practical Guide for Quantification of In Vivo Degradation Rates for Therapeutic Proteins with Single-Cell Resolution Using Fluorescence Ratio Imaging

Many tools for studying the pharmacokinetics of biologics lack single-cell resolution to quantify the heterogeneous tissue distribution and subsequent therapeutic degradation in vivo. This protocol describes a dual-labeling technique using two near-infrared dyes with widely differing residualization...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Pharmaceutics
Main Authors: Nessler, Ian, Cilliers, Cornelius, Thurber, Greg M.
Format: Artigo
Jezik:Inglês
Izdano: MDPI 2020
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7076450/
https://ncbi.nlm.nih.gov/pubmed/32033318
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/pharmaceutics12020132
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