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Single-Cell Quantification of Protein Degradation Rates by Time-Lapse Fluorescence Microscopy in Adherent Cell Culture

Proteins are in a dynamic state of synthesis and degradation and their half-lives can be adjusted under various circumstances. However, most commonly used approaches to determine protein half-life are either limited to population averages from lysed cells or require the use of protein synthesis inhi...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:J Vis Exp
Prif Awduron: Alber, Andrea Brigitta, Suter, David Michael
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: MyJove Corporation 2018
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC5912353/
https://ncbi.nlm.nih.gov/pubmed/29443092
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56604
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