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Measuring single-cell gene expression dynamics in bacteria using fluorescence time-lapse microscopy

Quantitative single-cell time-lapse microscopy is a powerful method for analyzing gene circuit dynamics and heterogeneous cell behavior. We describe the application of this method to imaging bacteria by using an automated microscopy system. This protocol has been used to analyze sporulation and comp...

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Hlavní autoři: Young, Jonathan W, Locke, James C W, Altinok, Alphan, Rosenfeld, Nitzan, Bacarian, Tigran, Swain, Peter S, Mjolsness, Eric, Elowitz, Michael B
Médium: Artigo
Jazyk:Inglês
Vydáno: 2011
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4161363/
https://ncbi.nlm.nih.gov/pubmed/22179594
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2011.432
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