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A Fluctuation Method to Quantify In Vivo Fluorescence Data

Quantitative in vivo measurements are essential for developing a predictive understanding of cellular behavior. Here we present a technique that converts observed fluorescence intensities into numbers of molecules. By transiently expressing a fluorescently tagged protein and then following its dilut...

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Detalhes bibliográficos
Main Authors: Rosenfeld, Nitzan, Perkins, Theodore J., Alon, Uri, Elowitz, Michael B., Swain, Peter S.
Formato: Artigo
Idioma:Inglês
Publicado em: Biophysical Society 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1483091/
https://ncbi.nlm.nih.gov/pubmed/16648159
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.105.073098
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