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A Fluctuation Method to Quantify In Vivo Fluorescence Data
Quantitative in vivo measurements are essential for developing a predictive understanding of cellular behavior. Here we present a technique that converts observed fluorescence intensities into numbers of molecules. By transiently expressing a fluorescently tagged protein and then following its dilut...
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Main Authors: | , , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
Biophysical Society
2006
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1483091/ https://ncbi.nlm.nih.gov/pubmed/16648159 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.105.073098 |
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