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Small molecule regulated sgRNAs enable control of genome editing in E. coli by Cas9

CRISPR-Cas9 has led to great advances in gene editing for a broad spectrum of applications. To further the utility of Cas9 there have been efforts to achieve temporal control over its nuclease activity. While different approaches have focused on regulation of CRISPR interference or editing in mammal...

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Detalles Bibliográficos
Publicado en:Nat Commun
Main Authors: Iwasaki, Roman S., Ozdilek, Bagdeser A., Garst, Andrew D., Choudhury, Alaksh, Batey, Robert T.
Formato: Artigo
Idioma:Inglês
Publicado: Nature Publishing Group UK 2020
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC7070018/
https://ncbi.nlm.nih.gov/pubmed/32170140
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-020-15226-8
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