Resolving dynamics and function of transient states in single enzyme molecules

We use a hybrid fluorescence spectroscopic toolkit to monitor T4 Lysozyme (T4L) in action by unraveling the kinetic and dynamic interplay of the conformational states. In particular, by combining single-molecule and ensemble multiparameter fluorescence detection, EPR spectroscopy, mutagenesis, and F...

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Detalhes bibliográficos
Publicado no:Nat Commun
Main Authors: Sanabria, Hugo, Rodnin, Dmitro, Hemmen, Katherina, Peulen, Thomas-Otavio, Felekyan, Suren, Fleissner, Mark R., Dimura, Mykola, Koberling, Felix, Kühnemuth, Ralf, Hubbell, Wayne, Gohlke, Holger, Seidel, Claus A. M.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2020
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7060211/
https://ncbi.nlm.nih.gov/pubmed/32144241
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-020-14886-w
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