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The resolution revolution in cryoEM requires high-quality sample preparation: a rapid pipeline to a high-resolution map of yeast fatty acid synthase

Single-particle electron cryo-microscopy (cryoEM) has undergone a ‘resolution revolution’ that makes it possible to characterize megadalton (MDa) complexes at atomic resolution without crystals. To fully exploit the new opportunities in molecular microscopy, new procedures for the cloning, expressio...

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Detalhes bibliográficos
Publicado no:IUCrJ
Main Authors: Joppe, Mirko, D’Imprima, Edoardo, Salustros, Nina, Paithankar, Karthik S., Vonck, Janet, Grininger, Martin, Kühlbrandt, Werner
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7055384/
https://ncbi.nlm.nih.gov/pubmed/32148850
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2052252519017366
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