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The resolution revolution in cryoEM requires high-quality sample preparation: a rapid pipeline to a high-resolution map of yeast fatty acid synthase

Single-particle electron cryo-microscopy (cryoEM) has undergone a ‘resolution revolution’ that makes it possible to characterize megadalton (MDa) complexes at atomic resolution without crystals. To fully exploit the new opportunities in molecular microscopy, new procedures for the cloning, expressio...

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Publicat a:IUCrJ
Autors principals: Joppe, Mirko, D’Imprima, Edoardo, Salustros, Nina, Paithankar, Karthik S., Vonck, Janet, Grininger, Martin, Kühlbrandt, Werner
Format: Artigo
Idioma:Inglês
Publicat: International Union of Crystallography 2020
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7055384/
https://ncbi.nlm.nih.gov/pubmed/32148850
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2052252519017366
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