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The resolution revolution in cryoEM requires high-quality sample preparation: a rapid pipeline to a high-resolution map of yeast fatty acid synthase

Single-particle electron cryo-microscopy (cryoEM) has undergone a ‘resolution revolution’ that makes it possible to characterize megadalton (MDa) complexes at atomic resolution without crystals. To fully exploit the new opportunities in molecular microscopy, new procedures for the cloning, expressio...

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Dettagli Bibliografici
Pubblicato in:IUCrJ
Autori principali: Joppe, Mirko, D’Imprima, Edoardo, Salustros, Nina, Paithankar, Karthik S., Vonck, Janet, Grininger, Martin, Kühlbrandt, Werner
Natura: Artigo
Lingua:Inglês
Pubblicazione: International Union of Crystallography 2020
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7055384/
https://ncbi.nlm.nih.gov/pubmed/32148850
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2052252519017366
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