The resolution revolution in cryoEM requires high-quality sample preparation: a rapid pipeline to a high-resolution map of yeast fatty acid synthase

Single-particle electron cryo-microscopy (cryoEM) has undergone a ‘resolution revolution’ that makes it possible to characterize megadalton (MDa) complexes at atomic resolution without crystals. To fully exploit the new opportunities in molecular microscopy, new procedures for the cloning, expressio...

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Bibliographic Details
Published in:IUCrJ
Main Authors: Joppe, Mirko, D’Imprima, Edoardo, Salustros, Nina, Paithankar, Karthik S., Vonck, Janet, Grininger, Martin, Kühlbrandt, Werner
Format: Artigo
Language:Inglês
Published: International Union of Crystallography 2020
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Research Papers
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7055384/
https://ncbi.nlm.nih.gov/pubmed/32148850
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2052252519017366
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