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The resolution revolution in cryoEM requires high-quality sample preparation: a rapid pipeline to a high-resolution map of yeast fatty acid synthase

Single-particle electron cryo-microscopy (cryoEM) has undergone a ‘resolution revolution’ that makes it possible to characterize megadalton (MDa) complexes at atomic resolution without crystals. To fully exploit the new opportunities in molecular microscopy, new procedures for the cloning, expressio...

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Detalles Bibliográficos
Publicado en:IUCrJ
Autores principales: Joppe, Mirko, D’Imprima, Edoardo, Salustros, Nina, Paithankar, Karthik S., Vonck, Janet, Grininger, Martin, Kühlbrandt, Werner
Formato: Artigo
Lenguaje:Inglês
Publicado: International Union of Crystallography 2020
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC7055384/
https://ncbi.nlm.nih.gov/pubmed/32148850
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2052252519017366
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