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Quantitative Determination of Dark Chromophore Population Explains the Apparent Low Quantum Yield of Red Fluorescent Proteins

[Image: see text] The fluorescence quantum yield of four representative red fluorescent proteins mCherry, mKate2, mRuby2, and the recently introduced mScarlet was investigated. The excited state lifetimes were measured as a function of the distance to a gold mirror in order to control the local dens...

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Detalhes bibliográficos
Publicado no:J Phys Chem B
Main Authors: Prangsma, Jord C., Molenaar, Robert, van Weeren, Laura, Bindels, Daphne S., Haarbosch, Lindsay, Stouthamer, Jente, Gadella, Theodorus W. J., Subramaniam, Vinod, Vos, Willem L., Blum, Christian
Formato: Artigo
Idioma:Inglês
Publicado em: American Chemical Society 2020
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7049984/
https://ncbi.nlm.nih.gov/pubmed/32011884
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.jpcb.9b10396
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