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Controlling Protein Surface Orientation by Strategic Placement of Oligo-Histidine Tags
[Image: see text] We report oriented immobilization of proteins using the standard hexahistidine (His(6))-Ni(2+):NTA (nitrilotriacetic acid) methodology, which we systematically tuned to give control of surface coverage. Fluorescence microscopy and surface plasmon resonance measurements of self-asse...
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| Veröffentlicht in: | ACS Nano |
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| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
American
Chemical Society
2017
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| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5618149/ https://ncbi.nlm.nih.gov/pubmed/28850777 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acsnano.7b03717 |
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