Controlling Protein Surface Orientation by Strategic Placement of Oligo-Histidine Tags

[Image: see text] We report oriented immobilization of proteins using the standard hexahistidine (His(6))-Ni(2+):NTA (nitrilotriacetic acid) methodology, which we systematically tuned to give control of surface coverage. Fluorescence microscopy and surface plasmon resonance measurements of self-asse...

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Detalhes bibliográficos
Publicado no:ACS Nano
Main Authors: Wasserberg, Dorothee, Cabanas-Danés, Jordi, Prangsma, Jord, O’Mahony, Shane, Cazade, Pierre-Andre, Tromp, Eldrich, Blum, Christian, Thompson, Damien, Huskens, Jurriaan, Subramaniam, Vinod, Jonkheijm, Pascal
Formato: Artigo
Idioma:Inglês
Publicado em: American Chemical Society 2017
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5618149/
https://ncbi.nlm.nih.gov/pubmed/28850777
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acsnano.7b03717
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