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I-Block: a simple Escherichia coli-based assay for studying sequence-specific DNA binding of proteins
We have developed a simple method called I-Block assay, which can detect sequence-specific binding of proteins to DNA in Escherichia coli. The method works by detecting competition between the protein of interest and RNA polymerase for binding to overlapping target sites in a plasmid-borne lacI prom...
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| Udgivet i: | Nucleic Acids Res |
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| Main Authors: | , , , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
2020
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7049694/ https://ncbi.nlm.nih.gov/pubmed/31980824 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa014 |
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