I-Block: a simple Escherichia coli-based assay for studying sequence-specific DNA binding of proteins

We have developed a simple method called I-Block assay, which can detect sequence-specific binding of proteins to DNA in Escherichia coli. The method works by detecting competition between the protein of interest and RNA polymerase for binding to overlapping target sites in a plasmid-borne lacI prom...

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Bibliographic Details
Published in:Nucleic Acids Res
Main Authors: Szentes, Sarolta, Zsibrita, Nikolett, Koncz, Mihály, Zsigmond, Eszter, Salamon, Pál, Pletl, Zita, Kiss, Antal
Format: Artigo
Language:Inglês
Published: Oxford University Press 2020
Subjects:
Methods Online
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7049694/
https://ncbi.nlm.nih.gov/pubmed/31980824
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa014
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