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Triplicate PCR reactions for 16S rRNA gene amplicon sequencing are unnecessary

Conventional wisdom holds that PCR amplification for sequencing should employ pooled replicate reactions to reduce bias due to jackpot effects and chimera formation. However, modern amplicon data analysis employs methods that may be less sensitive to such artifacts. Here we directly compare results...

詳細記述

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書誌詳細
出版年:Biotechniques
主要な著者: Marotz, Clarisse, Sharma, Anukriti, Humphrey, Greg, Gottel, Neil, Daum, Christopher, Gilbert, Jack A, Eloe-Fadrosh, Emiley, Knight, Rob
フォーマット: Artigo
言語:Inglês
出版事項: 2019
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC7030937/
https://ncbi.nlm.nih.gov/pubmed/31124709
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/btn-2018-0192
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