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Influence of PCR cycle number on 16S rRNA gene amplicon sequencing of low biomass samples
The objective of this study was to evaluate the effects of increased PCR cycle number on sequencing results from samples with low microbial biomass, including bovine milk, and murine pelage and blood. We hypothesized that subjecting DNA from such samples to higher PCR cycle numbers would increase 16...
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| 發表在: | J Microbiol Methods |
|---|---|
| Main Authors: | , , , , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
2020
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7454008/ https://ncbi.nlm.nih.gov/pubmed/32805370 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mimet.2020.106033 |
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