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Two-Dimensional Gel Electrophoresis to Resolve DNA Topoisomers

Agarose gel electrophoresis is one of the most straightforward techniques that can be used to differentiate between topoisomers of closed circular DNA molecules. Generally, the products of reactions that monitor the interconversion of DNA between negatively supercoiled and relaxed DNA or positively...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Methods Mol Biol
Main Authors: Gibson, Elizabeth G., Oviatt, Alexandria A., Osheroff, Neil
Format: Artigo
Jezik:Inglês
Izdano: 2020
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7012906/
https://ncbi.nlm.nih.gov/pubmed/31989511
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-0716-0323-9_2
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