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Two-Dimensional Gel Electrophoresis to Resolve DNA Topoisomers

Agarose gel electrophoresis is one of the most straightforward techniques that can be used to differentiate between topoisomers of closed circular DNA molecules. Generally, the products of reactions that monitor the interconversion of DNA between negatively supercoiled and relaxed DNA or positively...

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Detalles Bibliográficos
Publicado en:Methods Mol Biol
Autores principales: Gibson, Elizabeth G., Oviatt, Alexandria A., Osheroff, Neil
Formato: Artigo
Lenguaje:Inglês
Publicado: 2020
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC7012906/
https://ncbi.nlm.nih.gov/pubmed/31989511
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-0716-0323-9_2
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