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Identification of protein binding sites in genomic DNA by two-dimensional gel electrophoresis.

We describe a simple two-dimensional electrophoresis procedure to identify the recognition sites of DNA-binding proteins within large DNA molecules. Using this approach, we have mapped E. coli IHF (Integration Host Factor) binding sites within phage Lambda (48 kb) and phage Mu (39 kb) DNA. We are al...

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Bibliografische gegevens
Hoofdauteurs: Boffini, A, Prentki, P
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 1991
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC333888/
https://ncbi.nlm.nih.gov/pubmed/1827523
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