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An optimized workflow for single-cell transcriptomics and repertoire profiling of purified lymphocytes from clinical samples

Establishing clinically relevant single-cell (SC) transcriptomic workflows from cryopreserved tissue is essential to move this emerging immune monitoring technology from the bench to the bedside. Improper sample preparation leads to detrimental cascades, resulting in loss of precious time, money and...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Sci Rep
Hauptverfasser: Hanamsagar, Richa, Reizis, Timothy, Chamberlain, Mathew, Marcus, Robert, Nestle, Frank O., de Rinaldis, Emanuele, Savova, Virginia
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Publishing Group UK 2020
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7010687/
https://ncbi.nlm.nih.gov/pubmed/32042039
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-58939-y
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