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Improving sensitivity of single tube nested PCR to detect fastidious microorganisms

Single Tube Nested PCR (ST-nPCR) is of value to clinical laboratories with limited settings for the detection of fastidious microorganisms. The detection sensitivity of ST-nPCR is dependent on ensuring minimal leftovers of outer primers during the second round of the reaction. In this work, we inves...

Täydet tiedot

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Bibliografiset tiedot
Julkaisussa:Heliyon
Päätekijät: Shatleh-Rantisi, Diala, Tamimi, Asmaa, Ashhab, Yaqoub
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: Elsevier 2020
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC7002839/
https://ncbi.nlm.nih.gov/pubmed/32042975
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.heliyon.2020.e03246
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