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Single-tube, nested, reverse transcriptase PCR for detection of viable Mycobacterium tuberculosis.

Several problems remain before molecular biology-based techniques, such as PCR, are widely accepted for the detection of infectious agents. Among the most formidable of these problems are the inability of the tests to distinguish between viable and nonviable organisms. We approached this problem by...

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Detalhes bibliográficos
Publicado no:J Clin Microbiol
Principais autores: Jou, N T, Yoshimori, R B, Mason, G R, Louie, J S, Liebling, M R
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1997
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC232722/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9114400/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.35.5.1161-1165.1997
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