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Improving sensitivity of single tube nested PCR to detect fastidious microorganisms
Single Tube Nested PCR (ST-nPCR) is of value to clinical laboratories with limited settings for the detection of fastidious microorganisms. The detection sensitivity of ST-nPCR is dependent on ensuring minimal leftovers of outer primers during the second round of the reaction. In this work, we inves...
Gardado en:
| Publicado en: | Heliyon |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Elsevier
2020
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7002839/ https://ncbi.nlm.nih.gov/pubmed/32042975 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.heliyon.2020.e03246 |
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