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High cell density transient transfection of CHO cells for TGF‐β1 expression

High cell densities for transient transfection with polyethyleneimine (PEI) can be used for rapid and maximal production of recombinant proteins. High cell densities can be obtained by different cultivation systems, such as batch or perfusion systems. Herein, densities up to 18 million cells/mL were...

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Publicat a:Eng Life Sci
Autors principals: Elshereef, Abdalla A., Jochums, André, Lavrentieva, Antonina, Stuckenberg, Lena, Scheper, Thomas, Solle, Dörte
Format: Artigo
Idioma:Inglês
Publicat: John Wiley and Sons Inc. 2019
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6999575/
https://ncbi.nlm.nih.gov/pubmed/32624966
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/elsc.201800174
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