High cell density transient transfection of CHO cells for TGF‐β1 expression

High cell densities for transient transfection with polyethyleneimine (PEI) can be used for rapid and maximal production of recombinant proteins. High cell densities can be obtained by different cultivation systems, such as batch or perfusion systems. Herein, densities up to 18 million cells/mL were...

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Bibliographic Details
Published in:Eng Life Sci
Main Authors: Elshereef, Abdalla A., Jochums, André, Lavrentieva, Antonina, Stuckenberg, Lena, Scheper, Thomas, Solle, Dörte
Format: Artigo
Language:Inglês
Published: John Wiley and Sons Inc. 2019
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6999575/
https://ncbi.nlm.nih.gov/pubmed/32624966
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/elsc.201800174
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