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Engineering bi‐functional enzyme complex of formate dehydrogenase and leucine dehydrogenase by peptide linker mediated fusion for accelerating cofactor regeneration

This study reports the application of peptide linker in the construction of bi‐functional formate dehydrogenase (FDH) and leucine dehydrogenase (LeuDH) enzymatic complex for efficient cofactor regeneration and L‐tert leucine (L‐tle) biotransformation. Seven FDH‐LeuDH fusion enzymes with different pe...

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Bibliografske podrobnosti
izdano v:Eng Life Sci
Main Authors: Zhang, Yonghui, Wang, Yali, Wang, Shizhen, Fang, Baishan
Format: Artigo
Jezik:Inglês
Izdano: John Wiley and Sons Inc. 2017
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC6999412/
https://ncbi.nlm.nih.gov/pubmed/32624849
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/elsc.201600232
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