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Codon-Optimized NADH Oxidase Gene Expression and Gene Fusion with Glycerol Dehydrogenase for Bienzyme System with Cofactor Regeneration

NADH oxidases (NOXs) play an important role in maintaining balance of NAD(+)/NADH by catalyzing cofactors regeneration. The expression of nox gene from Lactobacillus brevis in Escherichia coli BL21 (BL21 (DE3)) was studied. Two strategies, the high AT-content in the region adjacent to the initiation...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Fang, Baishan, Jiang, Wei, Zhou, Qiang, Wang, Shizhen
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4482596/
https://ncbi.nlm.nih.gov/pubmed/26115038
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0128412
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