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Single-round isolation of diverse RNA aptamers from a random sequence pool

Aptamers are oligonucleotide ligands with specific binding affinity to target molecules. Generally, RNA aptamers are selected from an RNA pool with random sequences, using the technique termed SELEX, in which the target-binding RNA molecules are repeatedly isolated and exponentially amplified. Despi...

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Detalhes bibliográficos
Publicado no:Biol Methods Protoc
Main Authors: Imashimizu, Masahiko, Takahashi, Masaki, Amano, Ryo, Nakamura, Yoshikazu
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6994090/
https://ncbi.nlm.nih.gov/pubmed/32161798
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/biomethods/bpy004
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