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Localization microscopy at doubled precision with patterned illumination

MINFLUX offers a breakthrough in single molecule localization precision, but is limited in field-of-view. Here, we combine centroid estimation and illumination pattern induced photon count variations in a conventional widefield imaging setup to extract position information over a typical micron size...

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Detalhes bibliográficos
Publicado no:Nat Methods
Main Authors: Cnossen, Jelmer, Hinsdale, Taylor, Thorsen, Rasmus Ø., Siemons, Marijn, Schueder, Florian, Jungmann, Ralf, Smith, Carlas S., Rieger, Bernd, Stallinga, Sjoerd
Formato: Artigo
Idioma:Inglês
Publicado em: 2019
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6989044/
https://ncbi.nlm.nih.gov/pubmed/31819263
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41592-019-0657-7
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